Thana B.

Posted on Oct 6

Optimization of Red Blood Cell Shape Using Spherical Harmonics Parameterization

Introduction

Red blood cells (RBCs) exhibit a distinctive biconcave disc shape, essential for their role in gas exchange and traversing microvasculature. Modeling and optimizing this shape can provide valuable insights into the cell's mechanical properties and behaviors under physiological and pathological conditions.

This article presents a comprehensive mathematical model for optimizing the RBC shape using spherical harmonics parameterization under axial symmetry. We then provide a detailed Python implementation, explicitly mapping each mathematical expression to corresponding code blocks. The implementation utilizes the CasADi library for symbolic computations and numerical optimization.

Mathematical Formulation for RBC Shape Optimization with Fixed Posture and Symmetry

1. Decision Variables

Spherical Harmonics Coefficients (Axially Symmetric):

The shape of the RBC is represented using only the coefficients for axisymmetric spherical harmonics:

C_{L,0} \quad \text{for } L \in {0, 1, \ldots, L_{\text{max}}}

This reduces the number of decision variables significantly since only the coefficients corresponding to $K = 0$ are retained due to axial symmetry.

2. Objective Function

The objective is to minimize the total energy of the RBC membrane:

\text{Minimize} \quad E_{\text{total}}(C_{L,0}) = E_{\text{bending}}(C_{L,0}) + E_{\text{shear}}(C_{L,0}) + E_{\text{coupling}}(C_{L,0}) + E_{\text{viscous}}(C_{L,0}) + E_{\text{thermal}}(C_{L,0}) + E_{\text{hydro}}(C_{L,0})

Where each energy term is calculated using the reduced set of coefficients. The energy components are:

Bending Energy

E_{\text{bending}} = \sum_{i=1}^{N} \frac{k_b}{2} \left( 2H_i - C_0 \right)^2 \cdot A_i

$k_b$ : Bending modulus.
$H_i$ : Mean curvature at point $i$ .
$C_0$ : Spontaneous curvature (assumed zero in this model).
$A_i$ : Area element at point $i$ .

Shear Energy

E_{\text{shear}} = \sum_{i=1}^{N} \frac{K_s}{2} \left( \lambda_{1,i}^2 + \lambda_{2,i}^2 - 2 \right) \cdot A_i

$K_s$ : Shear modulus.
$\lambda_{1,i}, \lambda_{2,i}$ : Principal stretches at point $i$ .

Coupling Energy

E_{\text{coupling}} = \sum_{i=1}^{N} \gamma (H_i - C_0) \left( \lambda_{1,i}^2 + \lambda_{2,i}^2 \right) \cdot A_i

$\gamma$ : Coupling constant.

Viscous Energy

E_{\text{viscous}} = \sum_{i=1}^{N} \eta \left( \frac{\partial H_i}{\partial t} \right)^2 \cdot A_i

$\eta$ : Viscosity coefficient.
$\frac{\partial H_i}{\partial t}$ : Temporal change in curvature.

Thermal Fluctuation Energy

E_{\text{thermal}} = \sum_{i=1}^{N} F_{\text{thermal},i} \cdot A_i

$F_{\text{thermal},i}$ : Stochastic thermal fluctuation force at point $i$ .

Hydrodynamic Forces

E_{\text{hydro}} = \sum_{i=1}^{N} P_{\text{flow},i} \cdot \mathbf{n}_i \cdot A_i

$P_{\text{flow},i}$ : Pressure or shear force from fluid at point $i$ .
$\mathbf{n}_i$ : Normal vector at point $i$ .

3. Constraints

3.1 Volume Constraint

Ensure the RBC volume matches physiological values:

V(C_{L,0}) = \sum_{i=1}^{N} V_i = V_{\text{target}} \quad \text{(e.g., } V_{\text{target}} \approx 90 \, \mu\text{m}^3 \text{)}

3.2 Surface Area Constraint

Ensure the surface area remains constant:

A(C_{L,0}) = \sum_{i=1}^{N} A_i = A_{\text{target}} \quad \text{(e.g., } A_{\text{target}} \approx 145 \, \mu\text{m}^2 \text{)}

3.3 Area Difference Constraint

Account for the area difference between the inner and outer leaflets:

\Delta A(C_{L,0}) = \sum_{i=1}^{N} 2H_i \cdot A_i = \Delta A_{\text{target}} \quad \text{(e.g., } \Delta A_{\text{target}} \approx 0.6 - 1.0 \text{)}

3.4 Reduced Volume Constraint

Maintain a reduced volume less than 1 for a non-spherical shape:

v = \frac{V(C_{L,0})}{\left( \frac{4}{3} \pi \left( \frac{A(C_{L,0})}{4 \pi} \right)^{3/2} \right)} \quad \text{and } v \in [0.6, 0.7]

3.5 Symmetry Constraints

Impose symmetry on the coefficients:

C_{L,K} = 0 \quad \text{for all } K \neq 0

Python Implementation Using CasADi

We implement the mathematical model using Python and the CasADi library, which allows for symbolic differentiation and optimization. Below, each code block is mapped to the corresponding mathematical expression.

1. Import Necessary Libraries



import casadi as ca  # CasADi for symbolic computations
import numpy as np   # NumPy for numerical operations
import matplotlib.pyplot as plt  # For plotting
from mpl_toolkits.mplot3d import Axes3D  # For 3D plotting

2. Define Constants and Parameters

Set the physical constants, target values, and discretization parameters as per physiological data.



# Physical constants
k_b = 2e-19    # Bending modulus (J)
K_s = 5e-2     # Shear modulus (N/m), may be simplified
gamma = 1e-9   # Coupling constant
C_0 = 0        # Spontaneous curvature (assumed zero)

# Target values for constraints
V_target = 90e-18      # Target volume (m³)
A_target = 145e-12     # Target surface area (m²)
Delta_A_target = 0.85  # Target area difference
v_target = 0.7         # Target reduced volume

# Spherical harmonics parameters
L_max = 6    # Maximum degree for spherical harmonics

# Discretization parameters
N_theta = 200    # Number of discretization points for theta
theta_values = np.linspace(0, np.pi, N_theta)  # θ grid points
dtheta = theta_values[1] - theta_values[0]

3. Set Up the Optimization Problem

Decision Variables:

Mathematical Expression:

C_{L,0} \quad \text{for } L = 0 \text{ to } L_{\text{max}}

Code Implementation:



# Create an optimization problem
opt = ca.Opti()

# Define Theta as a parameter
theta_mx = opt.parameter(N_theta)
opt.set_value(theta_mx, theta_values)
cos_theta = ca.cos(theta_mx)
sin_theta = ca.sin(theta_mx)

# Define Decision Variables
C_L0_var = opt.variable(L_max + 1)  # Spherical harmonics coefficients

4. Compute Legendre Polynomials

Compute $P_L(\cos\theta)$ for $L = 0$ to $L_{\text{max}}$ .

Mathematical Expression:

P_L(\cos\theta)

Code Implementation:



# Function to compute Legendre polynomials iteratively
def legendre_P_iterative(L_max, x):
    P = []
    P.append(ca.MX.ones(x.shape))  # P0 = 1
    if L_max >= 1:
        P.append(x)  # P1 = x
        for L in range(2, L_max + 1):
            P_L = ((2 * L - 1) * x * P[L - 1] - (L - 1) * P[L - 2]) / L
            P.append(P_L)
    return P

# Compute Legendre Polynomials
P_L_list = legendre_P_iterative(L_max, cos_theta)

5. Shape Representation $r(\theta)$

Mathematical Expression:

r(\theta) = \sum_{L=0}^{L_{\text{max}}} C_{L,0} \, P_L(\cos\theta)

Code Implementation:



# Compute r(θ) symbolically
r_theta_var = C_L0_var[0] * P_L_list[0]
for L in range(1, L_max + 1):
    r_theta_var += C_L0_var[L] * P_L_list[L]



# Compute mean curvature H(θ)
numerator_var = (
    dr_dtheta_var**2
    - r_theta_var * d2r_dtheta2_var
    + r_theta_var * dr_dtheta_var * ca.tan(theta_mx)
)
denominator_var = (dr_dtheta_var**2 + r_theta_var**2) ** (1.5)
H_i_var = numerator_var / denominator_var

7. Define Energy Components

Bending Energy

Mathematical Expression:

E_{\text{bending}} = \sum_{i=1}^{N} \frac{k_b}{2} \left( 2H_i - C_0 \right)^2 A_i

Code Implementation:



# Bending Energy
E_bending_var = (k_b / 2.0) * ca.sum1((2.0 * H_i_var - C_0) ** 2 * dA_var)

Shear Energy

Assuming negligible shear deformation:

Mathematical Expression:

E_{\text{shear}} = 0

Code Implementation:



# Shear Energy (simplified)
E_shear_var = 0

Coupling Energy

Mathematical Expression:

E_{\text{coupling}} = \sum_{i=1}^{N} \gamma (H_i - C_0) \cdot A_i

Code Implementation:



# Coupling Energy
E_coupling_var = gamma * ca.sum1((H_i_var - C_0) * dA_var)

Total Energy

Mathematical Expression:

E_{\text{total}} = E_{\text{bending}} + E_{\text{shear}} + E_{\text{coupling}}

Code Implementation:



# Total Energy
E_total_var = E_bending_var + E_shear_var + E_coupling_var

8. Define Constraints

Volume Constraint

Mathematical Expression:

V = \sum_{i=1}^{N} V_i = V_{\text{target}}

Where:

V_i = 2\pi r_i^2 \sin\theta_i \Delta\theta

Code Implementation:



# Volume Constraint
V_var = 2 * np.pi * ca.sum1((r_theta_var**2) * sin_theta * dtheta)
opt.subject_to(V_var == V_target)

Surface Area Constraint

Mathematical Expression:

A = \sum_{i=1}^{N} A_i = A_{\text{target}}

Code Implementation:



# Surface Area Constraint
A_var = 2.0 * ca.pi * ca.sum1(r_theta_var * sin_theta * ca.sqrt(E_var) * dtheta)
opt.subject_to(A_var == A_target)

Reduced Volume Constraint

Mathematical Expression:

v = \frac{V}{\left( \frac{4}{3} \pi R_0^3 \right)} = v_{\text{target}}, \quad R_0 = \sqrt{\frac{A}{4\pi}}

Code Implementation:



# Reduced Volume Constraint
v_var = V_var / ((4.0 / 3.0) * ca.pi * (A_var / (4.0 * ca.pi)) ** 1.5)
opt.subject_to(v_var == v_target)

Area Difference Constraint

Mathematical Expression:

\Delta A = \sum_{i=1}^{N} 2H_i A_i = \Delta A_{\text{target}}

Code Implementation:



# Area Difference Constraint
Delta_A_var = ca.sum1(2.0 * H_i_var * dA_var)
opt.subject_to(Delta_A_var == Delta_A_target)

Symmetry Constraints

Only coefficients $C_{L,0}$ are considered, ensuring axial symmetry.

Code Implementation:

Implicitly enforced by only defining $C_{L,0}$ variables (no $C_{L,K}$ for $K \neq 0$ ).

Positivity Constraint for $r(\theta)$

Ensure physical validity of the shape.

Mathematical Expression:

r(\theta_i) > 0 \quad \forall i

Code Implementation:



# Ensure positive radius
opt.subject_to(r_theta_var > 0)

Biconcavity Enforcement

To ensure a biconcave shape, enforce $\frac{dr}{d\theta} < 0$ around $\theta = \frac{\pi}{2}$ .

Mathematical Expression:

\frac{dr}{d\theta} \bigg|_{\theta = \frac{\pi}{2}} < 0

Code Implementation:



# Enforce biconcavity around θ = π/2
delta_theta = np.pi / 20        # Range around θ = π/2
theta_mid = np.pi / 2
theta_lower = theta_mid - delta_theta
theta_upper = theta_mid + delta_theta

# Indices within specified θ range
theta_mid_indices = np.where(
    (theta_values >= theta_lower) & (theta_values <= theta_upper)
)[0]

epsilon = 1e-6  # Small positive value

# Enforce dr/dθ <= -epsilon in the specified θ range
for idx in theta_mid_indices:
    opt.subject_to(dr_dtheta_var[idx] <= -epsilon)

9. Set Objective and Solve

Code Implementation:



# Set the objective function
opt.minimize(E_total_var)

# Set initial guesses for coefficients
C_L0_initial = np.zeros(L_max + 1)
C_L0_initial[0] = bound_scale  # Approximate radius
opt.set_initial(C_L0_var, C_L0_initial)

# Set solver options
p_opts = {"expand": True}
s_opts = {"max_iter": 10000, "tol": 1e-10, "acceptable_tol": 1e-8}

# Specify solver
opt.solver("ipopt", p_opts, s_opts)

# Solve the optimization problem
try:
    sol = opt.solve()
except RuntimeError as e:
    print("Solver failed:", e)
    sol = opt.debug   # Retrieve solution even if solver fails

# Retrieve the optimal coefficients
C_L0_opt = sol.value(C_L0_var)

10. Post-processing and Visualization

Compute Optimized Shape

Mathematical Expression:

r_{\text{opt}}(\theta) = \sum_{L=0}^{L_{\text{max}}} C_{L,0}^{\text{opt}} \, P_L(\cos\theta)

Code Implementation:



# Compute the optimal shape r(θ) using the optimal coefficients
r_theta_opt = C_L0_opt[0] * P_L_list[0]
for L in range(1, L_max + 1):
    r_theta_opt += C_L0_opt[L] * P_L_list[L]
r_theta_opt = sol.value(r_theta_opt)

Generate 3D Coordinates

Convert $r_{\text{opt}}(\theta)$ to Cartesian coordinates for visualization.

Mathematical Expressions:

\begin{align*} x &= r(\theta) \sin\theta \cos\phi \ y &= r(\theta) \sin\theta \sin\phi \ z &= r(\theta) \cos\theta \end{align*}

Code Implementation:



# Additional discretization for φ
N_phi = 200
phi_values = np.linspace(0, 2 * np.pi, N_phi)
theta_grid, phi_grid = np.meshgrid(theta_values, phi_values)

# Replicate r(θ) across φ
r_grid = np.tile(r_theta_opt, (N_phi, 1))

# Compute the 3D coordinates (x, y, z)
x_coords = r_grid * np.sin(theta_grid) * np.cos(phi_grid)
y_coords = r_grid * np.sin(theta_grid) * np.sin(phi_grid)
z_coords = r_grid * np.cos(theta_grid)

Plot the Optimized Shape

Code Implementation:



# Plot the 3D shape
fig = plt.figure(figsize=(8, 8))
ax = fig.add_subplot(111, projection="3d")
ax.plot_surface(
    x_coords,
    y_coords,
    z_coords,
    rstride=4,
    cstride=4,
    color='red',
    edgecolor="none",
    alpha=0.8
)
ax.set_xlabel("x (m)")
ax.set_ylabel("y (m)")
ax.set_zlabel("z (m)")
ax.set_title("Optimized Red Blood Cell Shape (3D)")

# Ensure equal scaling on all axes
ax.set_box_aspect([np.ptp(x_coords), np.ptp(y_coords), np.ptp(z_coords)])

plt.tight_layout()
plt.show()

11. Validate Results

Ensure that the optimized shape satisfies the constraints.

Code Implementation:



# Compute final volume and area
V_final = sol.value(V_var)
A_final = sol.value(A_var)
v_final = sol.value(v_var)
Delta_A_final = sol.value(Delta_A_var)

print(f"Final Volume: {V_final:.3e} m³")
print(f"Target Volume: {V_target:.3e} m³")
print(f"Final Surface Area: {A_final:.3e} m²")
print(f"Target Surface Area: {A_target:.3e} m²")
print(f"Final Reduced Volume: {v_final:.3f}")
print(f"Target Reduced Volume: {v_target:.3f}")
print(f"Final Delta A: {Delta_A_final:.3e}")
print(f"Target Delta A: {Delta_A_target:.3e}")

Discussion

Mapping Mathematical Expressions to Code

In the above implementation, we meticulously map each mathematical expression from the model to corresponding code blocks. This explicit correspondence ensures that the code accurately reflects the theoretical formulations and that each component is correctly implemented.

Decision Variables: The spherical harmonics coefficients $C_{L,0}$ are represented by C_L0_var.
Shape Representation: The radial function $r(\theta)$ is constructed using the Legendre polynomials and the coefficients.
Geometric Quantities: Derivatives, mean curvature, and area elements are computed symbolically, matching the mathematical definitions.
Energy Components: The bending and coupling energies are calculated as per their mathematical expressions, and summed to form the total energy.
Constraints: Volume, surface area, reduced volume, area difference, and symmetry constraints are enforced using opt.subject_to.
Optimization: The total energy is minimized using IPOPT via CasADi.
Visualization: The optimized shape is visualized in 3D, providing a graphical representation of the results.
Validation: Final values are compared to target constraints to verify the optimization's success.

Simplifications and Assumptions

Shear Energy: Set to zero, assuming negligible shear deformation for simplicity.
Viscous, Thermal, and Hydrodynamic Energies: Omitted in this static optimization model.
Spontaneous Curvature ( $C_0$ ): Assumed zero, but can be adjusted if needed.

Advantages of Using CasADi

Symbolic Differentiation: Enables precise computation of derivatives required for curvature and energy calculations.
Optimization Framework: Provides tools to define variables, objectives, and constraints in an intuitive manner.
Solver Integration: Seamlessly integrates with IPOPT and other solvers for efficient numerical optimization.

Conclusion

This article presented a detailed mathematical model for optimizing the shape of a red blood cell using spherical harmonics parameterization under axial symmetry. By mapping each mathematical expression to corresponding code blocks, we provided a transparent and precise implementation.

The model minimizes the membrane's total energy while satisfying physiological constraints, resulting in an optimized biconcave shape consistent with actual RBCs. The approach demonstrates the power of combining mathematical modeling with computational tools like CasADi to explore and analyze complex biological systems.

Introduction

Mathematical Formulation for RBC Shape Optimization with Fixed Posture and Symmetry

1. Decision Variables

2. Objective Function

Bending Energy

Shear Energy

Coupling Energy

Viscous Energy

Thermal Fluctuation Energy

Hydrodynamic Forces

3. Constraints

3.1 Volume Constraint

3.2 Surface Area Constraint

3.3 Area Difference Constraint

3.4 Reduced Volume Constraint

3.5 Symmetry Constraints

Python Implementation Using CasADi

1. Import Necessary Libraries

2. Define Constants and Parameters

3. Set Up the Optimization Problem

Decision Variables:

4. Compute Legendre Polynomials

5. Shape Representation r(θ)r(\theta) r(θ)

6. Compute Geometric Quantities

First Derivative drdθ\frac{dr}{d\theta} dθdr​

Second Derivative d2rdθ2\frac{d^2r}{d\theta^2} dθ2d2r​

Metric Coefficient EE E

Area Element dAdA dA

Mean Curvature HH H

7. Define Energy Components

Bending Energy

Shear Energy

Coupling Energy

Total Energy

8. Define Constraints

Volume Constraint

Surface Area Constraint

Reduced Volume Constraint

Area Difference Constraint

Symmetry Constraints

Positivity Constraint for r(θ)r(\theta) r(θ)

Biconcavity Enforcement

9. Set Objective and Solve

10. Post-processing and Visualization

Compute Optimized Shape

Generate 3D Coordinates

Plot the Optimized Shape

11. Validate Results

Discussion

Mapping Mathematical Expressions to Code

Simplifications and Assumptions

Advantages of Using CasADi

Conclusion

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5. Shape Representation $r(\theta)$

First Derivative $\frac{dr}{d\theta}$

Second Derivative $\frac{d^2r}{d\theta^2}$

Metric Coefficient $E$

Area Element $dA$

Mean Curvature $H$

Positivity Constraint for $r(\theta)$